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Friday, December 23, 2011

Uncovering significant miRNA species with RNA-seq

Note to self: An easy way to uncover significant miRNA species with Illumina's RNA seq data is to run counts on the small RNA between two cell types, then toss out anything that appears ribosomal, filter for some minimum threshold and run a negative binomial distribution test on them like edge-R or DEseq. The resulting adjusted p-values can illuminate new miRNA species.

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